Education

1999-2003 B.S., Wuhan University, China

2003-2009 Ph.D., Wuhan University, China

Academic Experience

2005-2006 Research Fellow, Institute of Biotechnology, University of Helsinki, Finland

2009-2011 Scientific researcher, Lecturer (Post-doctoral), State Key Laboratory of Virology, College of life science, WuHan University, China

2011-2016 Scientific researcher, Associate Professor, State Key Laboratory of Virology, College of life science, WuHan University, China

2016- Scientific researcher, Professor, PI, Director of department of Virology, State Key Laboratory of Virology, College of life science, WuHan University, China

Awards & Honors

ELSEVIER Most Cited Chinese Researchers in the field of biology (2021)

ELSEVIER Most Cited Chinese Researchers in the field of biology (2022)

ELSEVIER Most Cited Chinese Researchers in the field of biology (2023)

Clarivate Highly Cited Researcher in the field of microbiology (2022)

Clarivate Highly Cited Researcher in the field of microbiology (2023)

Social Service

Deputy Chairman of the Youth Committee of the Virology Committee of the Chinese Society of Microbiology

Member of the Biosafety Expert Committee of the National Pathogenic Microbiology Laboratory

Expert of the guide compilation of the Ministry of Science and Technology

Expert of the guide compilation of the National Natural Science Foundation

Representative Publications

1. Wang H#, Feng J#, Fu Z, Xu T, Liu J, Yang S, Li Y, Deng J, Zhang Y, Guo M, Wang X, Zhang Z, Huang Z, Lan K, Zhou L, Chen Y*. Epitranscriptomic m5C methylation of SARS-CoV-2 RNA regulates viral replication and the virulence of progeny viruses in the new infection. Science Advances. 2024 Aug. In press.

2. Deng J#, Yang S#, Li Y#, Tan X, Liu J, Yu Y, Ding Q, Fan C, Wang H, Chen X, Liu Q, Guo X, Gong F, Zhou L, Chen Y. Natural evidence of coronaviral 2'-O-methyltransferase activity affecting viral pathogenesis via improved substrate RNA binding. Signal Transduct Target Ther. 2024 May 29;9(1):140. doi: 10.1038/s41392-024-01860-x.

3. Liu Q#, Lu Y#, Cai C#, Huang Y#, Zhou L, Guan Y, Fu S, Lin Y, Yan H, Zhang Z, Li X, Yang X, Yang H, Guo H*, Lan K*, Chen Y*, Hou SC*, Xiong Y*. A broad neutralizing nanobody against SARS-CoV-2 engineered from an approved drug. Cell Death Dis. 2024 Jun 28;15(6):458. doi: 10.1038/s41419-024-06802-7.

4. Zhang Z#, Zhou L#, Liu Q#, Zheng Y, Tan X, Huang Z, Guo M, Wang X, Chen X, Liang S, Li W, Song K, Yan K, Li J, Li Q, Zhang Y, Yang S, Cai Z, Dai M, Xian Q, Shi ZL, Xu K*, Lan K*, Chen Y*. The lethal K18-hACE2 knock-in mouse model mimicking the severe pneumonia of COVID-19 is practicable for antiviral development. Emerg Microbes Infect. 2024 Dec;13(1):2353302. doi: 10.1080/22221751.2024.2353302. Epub 2024 May 26.

5. Feng J#, Xu T#, He M, Li J, Yao P, Ma C, Yang S, Xu Z, Yan K, Chen X, Wang H, Liu J, Zeng C, Xia Y, Yan H, Zhou L*, Chen Y*. NSUN2-mediated m5C modification of HBV RNA positively regulates HBV replication. PLoS Pathog. 2023 Dec 4;19(12):e1011808. doi: 10.1371/journal.ppat.1011808.

6. Liu Q^#, Zhao H^#, Li Z^#, Zhang Z^#, Huang R^#, Gu M, Zhuang K, Xiong Q, Chen X, Yu W, Qian S, Zhang Y, Tan X, Zhang M, Yu F, Guo M, Huang Z, Wang X, Xiang W, Wu B, Mei F, Cai K, Zhou L, Zhou L, Wu Y*, Yan H*, Cao S*, Lan K*, Chen Y*. Broadly neutralizing antibodies derived from the earliest COVID-19 convalescents protect mice from SARS-CoV-2 variants challenge. Signal Transduct Target Ther. 2023 Sep 14;8(1):347. doi: 10.1038/s41392-023-01615-0. PMID: 37704615; PMCID: PMC10499932.

7. Wang H, Feng J, Zeng C, Liu J, Fu Z, Wang D, Wang Y, Zhang L, Li J, Jiang A, He M, Cao Y, Yan K, Tang H, Guo D, Xu K, Zhou X, Zhou L, Lan K, Zhou Y, Chen Y*. NSUN2-mediated M5c methylation of IRF3 mRNA negatively regulates type I interferon responses during various viral infections. Emerg Microbes Infect. 2023 Dec;12(1):2178238. doi: 10.1080/22221751.2023.2178238. PMID: 36748584; PMCID: PMC9946332.

8. Pan R^#, Kindler E^#, Cao L^#, Zhou Y, Zhang Z, Liu Q, Ebert N, Züst R, Sun Y, Gorbalenya AE, Perlman S*, Thiel V*, Chen Y*, Guo D*. N7-Methylation of the Coronavirus RNA Cap Is Required for Maximal Virulence by Preventing Innate Immune Recognition. mBio.2022;13(1):e0366221.Epub2022/01/26.doi: 10.1128/mbio.03662-21. PubMed PMID: 35073761; PMCID: PMC8787479.

9. Ma C^#, Chen X^#, Mei F^#, Xiong Q, Liu Q, Dong L, Liu C, Zou W, Zhan F, Hu B, Liu Y, Liu F, Zhou L, Xu J, Jiang Y, Xu K, Cai K*, Chen Y*, Yan H*, Lan K*. Drastic decline in sera neutralization against SARS-CoV-2 Omicron variant in Wuhan COVID-19 convalescents. Emerg Microbes Infect. 2022;11(1):567-572.

10. Liu Q^#, Xiong Q^#, Mei F^#, Ma C, Zhang Z, Hu B, Xu J, Jiang Y, Zhan F, Zhou S, Tao L, Chen X, Guo M, Wang X, Fang Y, Shen S, Liu Y, Liu F, Zhou L, Xu K, Ke C, Deng F, Cai K*, Yan H*, Chen Y*, Lan K*. Antibody neutralization to SARS-CoV-2 and variants after 1 year in Wuhan, China. Innovation (N Y). 2022;3(1):100181. Epub 2021/11/09. doi: 10.1016/j.xinn.2021.100181. PubMed PMID: 34746904; PMCID: PMC8563080.

11. Zhang Z^#, Liu Q^#, Sun Y, Li J, Liu J, Pan R, Cao L, Chen X, Li Y, Zhang Y, Xu K, Guo D, Zhou L, Lan K, Chen Y*. Live attenuated coronavirus vaccines deficient in N7-Methyltransferase activity induce both humoral and cellular immune responses in mice. Emerg Microbes Infect. 2021;10(1):1626-1637. Epub 2021/08/10. doi: 10.1080/22221751.2021.1964385. PubMed PMID: 34365904; PMCID: PMC8381960.

12. Yan H^#, Jiao H^#, Liu Q, Zhang Z, Xiong Q, Wang BJ, Wang X, Guo M, Wang LF, Lan K*, Chen Y*, Zhao H*. ACE2 receptor usage reveals variation in susceptibility to SARS-CoV and SARS-CoV-2 infection among bat species. Nature ecology & evolution. 2021;5(5):600-608. Epub 2021/03/03. doi: 10.1038/s41559-021-01407-1. PubMed PMID: 33649547.

13. Wang D^#, Jiang A^#, Feng J^#, Li G^#, Guo D^#, Sajid M, Wu K, Zhang Q, Ponty Y, Will S, Liu F, Yu X, Li S, Liu Q, Yang XL, Guo M, Li X, Chen M, Shi ZL, Lan K*, Chen Y*, Zhou Y*. The SARS-CoV-2 subgenome landscape and its novel regulatory features. Molecular cell. 2021;81(10):2135-2147.e5. Epub 2021/03/14. doi: 10.1016/j.molcel.2021.02.036. PubMed PMID: 33713597; PMCID: PMC7927579.

14. Yan K, Feng J, Liu X, Wang H, Li Q, Li J, Xu T, Sajid M, Ullah H, Zhou L, Zhou L*, Chen Y*. Inhibition of Hepatitis B Virus by AAV8-Derived CRISPR/SaCas9 Expressed From Liver-Specific Promoters. Frontiers in microbiology. 2021;12:665184. Epub 2021/07/14. doi: 10.3389/fmicb.2021.665184. PubMed PMID: 34254007; PMCID: PMC8271097.

15. Liu Y^#, Ning Z^#*, Chen Y^#*, Guo M^#, Liu Y, Gali NK, Sun L, Duan Y, Cai J, Westerdahl D, Liu X, Xu K, Ho KF*, Kan H*, Fu Q*, Lan K*. Aerodynamic analysis of SARS-CoV-2 in two Wuhan hospitals. Nature. 2020;582(7813):557-560. Epub 2020/04/28. doi: 10.1038/s41586-020-2271-3. PubMed PMID: 32340022.

16. Xiong Y^#, Liu Y^#, Cao L^#, Wang D^#, Guo M, Jiang A, Guo D, Hu W, Yang J, Tang Z, Wu H, Lin Y, Zhang M, Zhang Q, Shi M, Liu Y, Zhou Y*, Lan K*, Chen Y*. Transcriptomic characteristics of bronchoalveolar lavage fluid and peripheral blood mononuclear cells in COVID-19 patients. Emerg Microbes Infect. 2020;9(1):761-770. Epub 2020/04/02. doi: 10.1080/22221751.2020.1747363. PubMed PMID: 32228226; PMCID: PMC7170362.

17. Chen L^#, Liu W^#, Zhang Q^#, Xu K, Ye G, Wu W, Sun Z, Liu F, Wu K, Zhong B, Mei Y, Zhang W, Chen Y*, Li Y*, Shi M*, Lan K*, Liu Y*. RNA based mNGS approach identifies a novel human coronavirus from two individual pneumonia cases in 2019 Wuhan outbreak. Emerg Microbes Infect. 2020;9(1):313-329. Epub 2020/02/06. doi: 10.1080/22221751.2020.1725399. PubMed PMID: 32020836; PMCID: PMC7033720.

18. Suo T^#, Liu X^#, Feng J^#, Guo M^#, Hu W^#, Guo D, Ullah H, Yang Y, Zhang Q, Wang X, Sajid M, Huang Z, Deng L, Chen T, Liu F, Xu K, Liu Y, Zhang Q, Liu Y, Xiong Y*, Chen G*, Lan K*, Chen Y*. ddPCR: a more accurate tool for SARS-CoV-2 detection in low viral load specimens. Emerg Microbes Infect. 2020;9(1):1259-1268. Epub 2020/05/23. doi: 10.1080/22221751.2020.1772678. PubMed PMID: 32438868; PMCID: PMC7448897.

19. Liu X^#, Feng J^#, Zhang Q^#, Guo D^#, Zhang L, Suo T, Hu W, Guo M, Wang X, Huang Z, Xiong Y, Chen G*, Chen Y*, Lan K*. Analytical comparisons of SARS-COV-2 detection by qRT-PCR and ddPCR with multiple primer/probe sets. Emerg Microbes Infect. 2020;9(1):1175-1179. Epub 2020/05/26. doi: 10.1080/22221751.2020.1772679. PubMed PMID: 32448084; PMCID: PMC7448863.

20. Chen Y*, Liu Q, Guo D*. Emerging coronaviruses: Genome structure, replication, and pathogenesis. J Med Virol. 2020;92(4):418-423. Epub 2020/01/23. doi: 10.1002/jmv.25681. PubMed PMID: 31967327; PMCID: PMC7167049.

21. Yan L, Ge J, Zheng L, Zhang Y, Gao Y, Wang T, Huang Y, Yang Y, Gao S, Li M, Liu Z, Wang H, Li Y, Chen Y, Guddat LW, Wang Q, Rao Z*, Lou Z*. Cryo-EM Structure of an Extended SARS-CoV-2 Replication and Transcription Complex Reveals an Intermediate State in Cap Synthesis. Cell. 2021;184(1):184-193.e10. Epub 2020/11/25. doi: 10.1016/j.cell.2020.11.016. PubMed PMID: 33232691; PMCID: PMC7666536.

22. Cao L, Ji Y, Zeng L, Liu Q, Zhang Z, Guo S, Guo X, Tong Y, Zhao X, Li CM, Chen Y*, Guo D*. P200 family protein IFI204 negatively regulates type I interferon responses by targeting IRF7 in nucleus. PLoS Pathog. 2019;15(10):e1008079. Epub 2019/10/12. doi: 10.1371/journal.ppat.1008079. PubMed PMID: 31603949; PMCID: PMC6818788.

23. Gui M, Liu X, Guo D, Zhang Z, Yin CC*, Chen Y*, Xiang Y*. Electron microscopy studies of the coronavirus ribonucleoprotein complex. Protein & cell. 2017;8(3):219-224. Epub 2017/01/04. doi: 10.1007/s13238-016-0352-8. PubMed PMID: 28044277; PMCID: PMC5326623.

24. Chen Y*, Guo D*. Molecular mechanisms of coronavirus RNA capping and methylation. Virol Sin. 2016;31(1):3-11. Epub 2016/02/06. doi: 10.1007/s12250-016-3726-4. PubMed PMID: 26847650; PMCID: PMC7091378.

25. Li S, Zhu M, Pan R, Fang T, Cao YY, Chen S, Zhao X, Lei CQ, Guo L, Chen Y, Li CM, Jokitalo E, Yin Y, Shu HB, Guo D*. The tumor suppressor PTEN has a critical role in antiviral innate immunity. Nat Immunol. 2016;17(3):241-9. Epub 2015/12/23. doi: 10.1038/ni.3311. PubMed PMID: 26692175.

26. Cui L, Wang H, Ji Y, Yang J, Xu S, Huang X, Wang Z, Qin L, Tien P, Zhou X, Guo D*, Chen Y*. The Nucleocapsid Protein of Coronaviruses Acts as a Viral Suppressor of RNA Silencing in Mammalian Cells. J Virol. 2015;89(17):9029-43. Epub 2015/06/19. doi: 10.1128/jvi.01331-15. PubMed PMID: 26085159; PMCID: PMC4524063.

27. Liu X^#, Hao R^#, Chen S, Guo D, Chen Y*. Inhibition of hepatitis B virus by the CRISPR/Cas9 system via targeting the conserved regions of the viral genome. The Journal of general virology. 2015;96(8):2252-2261. Epub 2015/04/24. doi: 10.1099/vir.0.000159. PubMed PMID: 25904148.

28. Wang Y^#, Sun Y^#, Wu A, Xu S, Pan R, Zeng C, Jin X, Ge X, Shi Z, Ahola T, Chen Y*, Guo D*. Coronavirus nsp10/nsp16 Methyltransferase Can Be Targeted by nsp10-Derived Peptide In Vitro and In Vivo To Reduce Replication and Pathogenesis. J Virol. 2015;89(16):8416-27. Epub 2015/06/05. doi: 10.1128/jvi.00948-15. PubMed PMID: 26041293; PMCID: PMC4524257.

29. Wu A, Wang Y, Zeng C, Huang X, Xu S, Su C, Wang M, Chen Y*, Guo D*. Prediction and biochemical analysis of putative cleavage sites of the 3C-like protease of Middle East respiratory syndrome coronavirus. Virus Res. 2015;208:56-65. Epub 2015/06/04. doi: 10.1016/j.virusres.2015.05.018. PubMed PMID: 26036787; PMCID: PMC7114542.

30. Sun Y, Wang Z, Tao J, Wang Y, Wu A, Yang Z, Wang K, Shi L, Chen Y*, Guo D*. Yeast-based assays for the high-throughput screening of inhibitors of coronavirus RNA cap guanine-N7-methyltransferase. Antiviral Res. 2014;104:156-64. Epub 2014/02/18. doi: 10.1016/j.antiviral.2014.02.002. PubMed PMID: 24530452; PMCID: PMC7119097.

31. Chen Y, Tao J, Sun Y, Wu A, Su C, Gao G, Cai H, Qiu S, Wu Y, Ahola T, Guo D*. Structure-function analysis of severe acute respiratory syndrome coronavirus RNA cap guanine-N7-methyltransferase. J Virol. 2013;87(11):6296-305. Epub 2013/03/29. doi: 10.1128/jvi.00061-13. PubMed PMID: 23536667; PMCID: PMC3648086.

32. Chen Y^#, Su C^#, Ke M, Jin X, Xu L, Zhang Z, Wu A, Sun Y, Yang Z, Tien P, Ahola T, Liang Y, Liu X*, Guo D*. Biochemical and structural insights into the mechanisms of SARS coronavirus RNA ribose 2'-O-methylation by nsp16/nsp10 protein complex. PLoS Pathog. 2011;7(10):e1002294. Epub 2011/10/25. doi: 10.1371/journal.ppat.1002294. PubMed PMID: 22022266; PMCID: PMC3192843.

33. Chen Y, Cai H, Pan J, Xiang N, Tien P, Ahola T, Guo D*. Functional screen reveals SARS coronavirus nonstructural protein nsp14 as a novel cap N7 methyltransferase. Proc Natl Acad Sci U S A. 2009;106(9):3484-9. Epub 2009/02/12. doi: 10.1073/pnas.0808790106. PubMed PMID: 19208801; PMCID: PMC2651275.

34. Ke M^#, Chen Y^#, Wu A, Sun Y, Su C, Wu H, Jin X, Tao J, Wang Y, Ma X, Pan JA, Guo D*. Short peptides derived from the interaction domain of SARS coronavirus nonstructural protein nsp10 can suppress the 2'-O-methyltransferase activity of nsp10/nsp16 complex. Virus Res. 2012;167(2):322-8. Epub 2012/06/05. doi: 10.1016/j.virusres.2012.05.017. PubMed PMID: 22659295; PMCID: PMC7114426.

35. Hussain S, Pan J, Chen Y, Yang Y, Xu J, Peng Y, Wu Y, Li Z, Zhu Y, Tien P, Guo D*. Identification of novel subgenomic RNAs and noncanonical transcription initiation signals of severe acute respiratory syndrome coronavirus. J Virol. 2005;79(9):5288-95. Epub 2005/04/14. doi: 10.1128/jvi.79.9.5288-5295.2005. PubMed PMID: 15827143; PMCID: PMC1082772.