教育经历

2001.09-2005.07 上海交通大学，生物技术，学士

2005.09-2011.07 北京大学，生物技术专业，博士

2007.12-2009.12 日本国际农业研究中心，病毒学，联合培养博士

工作经历与任职

2011.09-2015.03 北京大学BIOPIC，博士后

2012.03-2012.09 美国哈佛大学化学与化学生物系，访问学者

2015.04-2018.12 北京大学BIOPIC，助理研究员

2019.02- 现在 武汉大学医学研究院/免疫与代谢前沿科学中心，教授

2019.08- 现在 武汉大学口腔医院，兼聘教授

获奖与荣誉

国家青年拔尖人才（2022）

湖北省楚天学者特聘教授 （2022）

湖北省杰出青年项目 （2022）

学术兼职

mSystems 学术编辑（2025.07–）

研究方向

病原微生物学：细菌耐药性、细菌慢性感染与免疫逃逸

成果概述

普颖颖实验室致力于持留菌（Persister）的研究。持留菌是具有生长潜能的休眠细菌亚群，是导致抗生素治疗失败和慢性感染复发的关键因素。实验室聚焦两大核心方向：持留菌的耐药机制与慢性感染的形成与维持。我们取得了一系列重要成果：1）首次发现细菌通过相分离形成无膜细胞器aggresome，并揭示其调控细菌抗生素耐药性的新机制；2）创新发展细菌单细胞技术，深入解析持留菌形成、耐受药物及复苏激活的分子过程；3）以结核分枝杆菌为模型，初步揭示了细菌在慢性感染过程中实现免疫逃逸的相关分子基础。 未来，实验室将继续深化研究持留菌介导慢性感染及逃逸宿主免疫的精细分子机制，并探索其潜在的临床干预策略与意义。

代表性论文

1. Pei L, Xian Y, Yan X, Schaefer C, Syeda A, Howard J, Zhang W, Liao H, Bai F*, Leake M*, Pu Y*. Aggresomes protect mRNA under stress in Escherichia coli. Nature Microbiology, Accepted in Principle. (2025).

2. Yan X, Liao H, Wang C, Huang C, Zhang W, Guo C, Pu Y*. An improved bacterial single-cell RNA-seq reveals biofilm heterogeneity. Elife. 2024 Dec 17;13:RP97543. doi: 10.7554/eLife.97543. PMID: 39689163; PMCID: PMC11651652.

3. Liao H, Yan X, Wang C, Huang C, Zhang W, Xiao L, Jiang J, Bao Y, Huang T, Zhang H, Guo C, Zhang Y, Pu Y*. Cyclic di-GMP as an antitoxin regulates bacterial genome stability and antibiotic persistence in biofilms. Elife. 2024 Oct 4;13:RP99194.

doi: 10.7554/eLife.99194. PMID: 39365286; PMCID: PMC11452175.

4. Zhou Y., Liao H., Pei L., and Pu Y* (2023). Combatting persister cells: The daunting task in post-antibiotics era. Cell Insight 2, 100104. 10.1016/j.cellin.2023.100104.

5. Zhang P^#, Qiu Y^#, Wang Y^#, Xiao Y, Yu S, Shi M, Ni Y, Miron R J, Pu Y*, Zhang Y*  (2022) Nanoparticles Promote Bacterial Antibiotic Tolerance via Inducing Hyperosmotic Stress Response，Small, https://doi.org/10.1002/smll.202105525

6. Qiu Y^#, Yu S^#, Wang Y^#, Xiao L, Pei L, Pu Y*, Zhang Y*. (2022) Photothermal Therapy may be a Double-edge Sword by Inducing the Formation of Bacterial Antibiotic Tolerance. Biomaterials Science, 00，1-11.

7. Liu Z^#, Wang A^#, Pu Y^#, Li Z, Xue D, Zhang C, Xiang X, E J, Bu Z, Bai F & Ji J. (2021) Genomic and Transcriptomic Profiling of Hepatoid Adenocarcinoma of the Stomach. Oncogene, 40, 5705–5717.

8. Tong, Y.*, ^# , Zhang, J.^#, Sun, N. ^#, Wang, X. ^#, Wei, Q., Zhang, Y., Huang, R., Pu, Y., Dai, H., Ren, B., Pei, G., Song, F., Zhu, G., Wang, X., Xia, X., Chen, X., Jiang, L., Zhang, J., Ouyang, L., Zhang, B., Jiang, Y., Liu, X., Calderone, R., Bai, F.*, Zhang, L.*,

Alterovitz, G. Berberine reverses multidrug resistance in Candida albicans by hijacking the drug efflux pump Mdr1p. Science Bulletin. 66, 1895-1905. (2021).

9. Mancini L, Terradot G, Tian T, Pu Y, Li Y, Lo C, Bai F and Pilizota T. (2020) A General Workflow for Characterization of Nernstian Dyes and Their Effects on Bacterial Physiology. Biophysical Journal, 117, 4–14.

10. Pu Y, Li Y, Bai F, et al. (2019). ATP-dependent dynamic protein aggregation regulates bacterial dormancy depth critical for antibiotic tolerance. Molecular Cell, 73(1):143-156.

11. Pu Y, Ke Y, Bai F. (2017). Active efflux in dormant bacterial cells - New insights into antibiotic persistence. Drug Resist Updat 30, 7-14.

12. Jin L, Qin Q, Wang Y, Pu Y, Li Y, et al. (2017). Rice Dwarf Virus P2 Protein Hijacks Auxin Signaling by Directly Targeting the Rice OsIAA10 Protein, Enhancing Viral Infection and Disease Development. PLoS Pathogens 12(9):e1005847.

13. Pu Y, Zhao Z, Li Y, Bai F, Xie X, et al. (2016). Enhanced Efflux Activity Facilitates Drug Tolerance in Dormant Bacterial Cells. Molecular Cell, 62, 284-294.

(Highlighted by Nature https://www.nature.com/articles/nature18442 and Nature Review Microbiology https://www.nature.com/articles/nrmicro.2016.72)

14. Guo X, Jin X, Lv X, Pu Y, Bai F*. (2015) Real Time Visualization of Perylene Nano-clusters (PNCs) in Water and Their Partitioning to Graphene Surface and Macrophage Cells. Environmental Science & Technology, 49 (13): 7926-7933.

15. Liu Y, Yang J, Zhao Z, Pu Y*, Bai F*. (2014) Bacterial Persistence. Science China Chemistry, 57 (12): 1625-1633

16. Pu Y, Kikuchi A, Moriyasu Y, Tomara M, Jin Y, Suga H, Wei T, Li Y, Omura T. (2011) Rice dwarf viruses with dysfunctional genomes generated in plants are filtered out in vector insects -implications for the virus origin. Journal of Virology, 85 (6): 2975-2979.

17. Akita F, Higashiura A, Shimizi T, Pu Y, Suzuki M, Ichiki T, Sasaya T, Kanamaru S, Arisaka F, Tsukihara T, Nakagawa A, Omura T. (2012) Crystal Structure and Octamerization of the Viroplasm Matrix Protein P9-1 of Rice Black Streaked Dwarf Virus. 

Journal of Virology, 86 (2): 746-756.

18. Zhou F, Pu Y, Wei T, Liu H, Deng W, Wei CH, Ding B, Omura T, Li Y. (2007). The P2 capsid protein of the nonenveloped rice dwarf phytoreovirus induces membrane fusionin insect host cells. Proceedings of the National Academy of Sciences USA. 104(49): 19547-19552.

19. Zhou F, Wu G, Deng W, Pu Y, Wei C, Li Y. (2007). Interaction of rice dwarf virus outer capsid P8 protein with rice glycolate oxidase mediates relocalization of P8. Febs Letters 581(1): 34-40.